Description

Description

PCR Cloning & Subcloning
PCR cloning and subcloning are two primary methods for amplifying DNA sequences. PCR cloning mimics the natural replication process of DNA, enabling the amplification and purification of DNA fragments in vitro. Subcloning involves selecting a fragment of DNA from cloned products and inserting it into a new vector. These techniques are widely applied in gene cloning, gene expression analysis, and other fields.

Synbio Technologies, leveraging the integration of DNA synthesis tools and cloning technologies, offers comprehensive PCR cloning and subcloning services. Our services are capable of cloning the target gene into any desired location of the vector, fulfilling your specific design requirements. For subcloning the same gene fragment into different vectors, we also provide efficient services to ensure the smooth progress of your downstream experiments.

Highlights
  • Fast Turnaround Time
  • 100% Sequence Accuracy
  • Independent of Vector Restriction Sites
  • Professional Technical Team
Service Details
Service TypeFragment LengthTurnaround Time (Business Days)Price
PCR Cloning & Subcloning<2 kb5-10Starting from $75
2-3 kb5-10
>3 kbQuote

* The turnaround time applies to non-complex sequences. Please contact quote@synbio-tech.com to quote for the complex sequences.

* Note

DNA Sequence

If the sequence has not been verified, we will provide Sanger sequencing verification. (Additional fees apply)

Specific Vectors

1. A Full-length sequence is required.

2. If there is no sequence information, we will provide Sanger sequencing verification. (Additional fees apply)

Rare RE or Special Molecular Biological Reagents

1. Customers will provide these reagents.

2. Synbio Technologies will purchase them for you (Additional fees apply) and return the excess after the delivery.

Workflow
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PCR Cloning & Subcloning
PCR Cloning & Subcloning
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